Chelsea youngsters Tammy Abraham, Isaiah Brown, Nathaniel Chalobah and Lewis Baker have all been included.
Meanwhile there are also places for Arsenal defender Rob Holding and Tottenham starlet Harry Winks.
Skipper James Ward-Prowse however, has been left out after being called up to Gareth Southgate’s senior squad.
The midfielder has impressed for Southampton this season, scoring two goals and assisting four in 20 Premier League appearances.
Boothroyd’s side face trips to Germany and Denmark during the international break later this month.